日本血吸蟲表皮抗原及候選疫苗基因、蛋白及其應用的制作方法

專利名稱：日本血吸蟲表皮抗原及候選疫苗基因、蛋白及其應用的制作方法
技術領域：
本發明涉及基因工程技術領域，具體地說涉及一類表達于日本血吸蟲表皮的蛋白質、其編碼核苷酸和編碼氨基酸序列以及其作為藥物靶點和疫苗的作用。
背景技術：
目前研究表明，血吸蟲病由血吸蟲的感染引起，在世界上有76個國家，受感染者約1.5億。致病的血吸蟲有3種，既日本血吸蟲(Schistosoma japonicum)，曼氏血吸蟲(S.mansoni)和埃及血吸蟲(S.haematobium)。在我國流行的是日本血吸蟲。
研制新的治療血吸蟲病和抗血吸蟲的藥物，首先必須找到合適的藥物作用靶點。血吸蟲的表皮是宿主和血吸蟲相互作用的界面。而組成血吸蟲表皮的蛋白質暴露在這個界面上，對小分子的化學藥物和大分子的抗體藥物而言都是最理想的藥物作用靶點。已經有研究表明治療血吸蟲的化學藥物——比喹酮的作用靶點就是存在于血吸蟲的表皮。另外一些已知的血吸蟲表皮蛋白，例如GST28，paramyosin，已經用于疫苗研究和應用。

發明內容
本發明要解決的技術問題是提供一類表達于日本血吸蟲表皮的蛋白質、其編碼核苷酸、及編碼氨基酸序列日本血吸蟲表皮蛋白，作為可能的藥物作用靶點和候選疫苗方面的作用。
為解決上述技術問題，本發明提供一種DNA序列，它具有序列表中SEQ IDNO.1～SEQ ID NO.23所述序列中任一序列的堿基序列；或者它是在中度嚴緊條件下與序列表中SEQ ID NO.1～SEQ ID NO.23所示的序列中任一條序列雜交的序列；或者它是與序列表中SEQ ID NO.1～SEQ ID NO.23所示的序列中任一條序列有至少70％同源性的序列。
為解決上述技術問題，本發明還提供一種氨基酸序列，它具有序列表中SEQID NO.24～SEQ ID NO.46所述序列中任一序列的氨基酸序列；或者它是與序列表中SEQID NO.24～SEQID NO.46所示的序列中任一條序列有至少70％同源性的序列；或者它是包含有序列表中SEQ ID NO.24～SEQ ID NO.46所示的序列中任一條序列的氨基酸序列。
本發明由于通過大規模的蛋白質組學研究，發現了23個日本血吸蟲表皮蛋白質，這些蛋白質，都是可能的藥物作用靶點和候選疫苗。血吸蟲病的流行給人民身體健康和經濟發展都造成巨大的損失，因此，防治血吸蟲病、開發研制治療血吸蟲病和抗血吸蟲的藥物具有巨大的社會和經濟效益。


圖1是免疫熒光抗體原位雜交實驗結果。
具體實施例方式
下面結合附圖和具體實施例對本發明作進一步詳細的說明。
實施例1日本血吸蟲表皮蛋白的分離和鑒定實驗1.日本血吸蟲表皮的分離純化血吸蟲表皮的純化按照文獻報道的Triton-x-100提取方法進行(參見Oaks，J.A.等人，寄生蟲學雜志，69(3)519-533(1983)。)。表皮蛋白從血吸蟲雄蟲、雌蟲、雌雄混合成蟲和童蟲分離。蟲體用Krebs’-Ringer’s，Tris-馬來酸鹽-緩沖鹽液(KRTM)洗3次，再用KRTM/0.2％Triton X-100溶液懸浮。這個液體和蟲體的混和物置于冰水浴中，在搖床上搖動10分鐘。然后，裝有液體和蟲體的混和物的試管在渦旋混合器上混旋幾次，然后再放回冰水浴中，靜置使蟲體下沉。用吸管吸出上部液體，放置到另一個新管中。這個試管在離心機上用2500×g的離心力，離心15分鐘。得到的沉淀就是血吸蟲的表皮。Krebs’-Ringer’s溶液的配方是0.15M NaCl，6.2mM KCl，1.2mM Na2HPO4，1.2mM MgSO4，10mM Glucose and 20mM Tris-Hepes，pH7.4。Tris maleate緩沖溶液的配方是0.2M Tris and 0.2MMaleicanhyclride，pH7.4。
2.日本血吸蟲表皮蛋白的電泳分離日本血吸蟲表皮用裂解劑裂解，蛋白溶液用SDS-聚丙烯酰胺凝膠電泳分離，條帶切割后再做第二維的在線毛細液相色譜分離以及質譜鑒定。軟件分析得到鑒定蛋白。分離好的血吸蟲表皮，用SDS-聚丙烯酰胺凝膠電泳(SDS-PAGE)緩沖液懸浮，用超聲波促進組織細胞裂解和蛋白溶解，超聲波裂解方法為超聲1秒，暫停3秒，總時間2分鐘。并用100℃加熱5分鐘以使蛋白質變性。然后這些表皮蛋白混合物用SDS-PAGE分離，分離方法按照普通的SDS凝膠電泳方法進行。膠上電泳泳道用刀切割下來，再用刀均等分割成8-12份。接下來做蛋白的膠內酶解。
3.日本血吸蟲表皮蛋白的膠內酶解過程膠內酶解參照文獻(Shevchenko，A.等人，化學年報，68850-858(1996)。)的方法進行。蛋白膠塊用刀切碎，每個小塊大小約為1立方毫米。加入100mMNH4HCO3，洗2次；轉入到試管中；加入10mM二硫蘇糖醇(dithiothreitol，DTT)/100mM NH4HCO3，56度放置反應1小時；冷卻到室溫，離心吸去DTT；加入等體積的55mM碘乙酰胺iodoacetamide/100mM NH4HCO3，室溫/黑暗反應45分鐘；吸去iodoacetamide，加入100mM NH4HCO3，洗2次；b去掉NH4HCO3，加入乙腈縮膠；去掉乙腈，真空抽干；加入100mM NH4HCO3，膠膨脹，去掉NH4HCO3，加入乙腈縮膠；去掉乙腈，抽干；加入含Trypsin的消化緩沖液(50mM NH4HCO3/12.5ng/ul trypsin)冰上放置45min；去掉多余酶液，加入50mM NH4HCO3，以剛覆蓋膠為準；37度，過夜酶切；酶切結束后，從試管中吸出上清液到新管子中，膠中加入60％ACN/0.5％HAC，在水浴中超聲處理15min，再重復一次；吸出上清液到新管子中，在原管子中再加入60％ACN/0.5％HAC，37度40min，吸出上清液，與前面的上清液合并，真空抽氣去ACN。
4.日本血吸蟲表皮蛋白酶解肽段的在線色譜分離和質譜鑒定酶解產生的肽混合物，用真空將溶液體積抽減到30μl。之后，每個組分的肽混合物上樣到一個反相捕獲柱(C18，5μm，300，300μm i.d×5mm，LC Packings)，在20μl/min的流速下脫鹽。然后這個捕獲柱和一個分析用的75μm×150mm C18柱(LC Packings)相連接，肽混合物就在200nl/min的流速下，從這個柱子上洗脫進入裝有Protana NanoES的電噴霧離子源的QSTAR pulser i中。Agilent1100毛細液相系統(Agilent Technologies)用來提供流動相A(0.5％乙酸/水)和流動相B(0.5％乙酸/乙腈)，線性梯度是60分鐘內5％B到50％B，30分鐘內50％B到90％B，然后是在90％B停留15分鐘。在分析柱后通過一個不銹鋼的兩通(Valco Instrument)連接一個10-μm直徑的PicoTip納噴霧頭(New Objective)，加上2500-3000伏的噴霧電壓，以獲得穩定的噴霧。MS/MS串聯質譜譜圖通過信息依賴的采集(IDA)和任務-循環增強來記錄。在每個測定掃描中，選取3-6個信號最強、帶2到3個電荷的離子，用滾動碰撞能量打碎，以獲得串聯質譜的信息。
5.日本血吸蟲表皮蛋白的軟件搜索鑒定用牛胰酶的自裂解片段如([M+H]+)1153.5741，2163.0569和2274.1600作為內標，或者以預先數據庫搜索得到的得分高的肽段的分子量，來校正所有的質譜譜圖。質譜搜索用的數據庫，包括從8420個從EST cluster預測得到的血吸蟲蛋白質序列，以及可能的污染蛋白質序列如牛胰酶的序列、宿主蛋白污染如人蛋白質序列、小鼠蛋白質序列、大鼠蛋白質序列、兔子蛋白質序列和角蛋白序列等。搜索軟件是MASCOT(http://www.matrixscience.com/，MatrixScience)。搜索參數為最大漏切點，3個；monoisotopic；肽電荷數，+1/+2/+3；修飾，半胱氨酸carbamoylmethylation和methionine氧化。搜索結果合并后，用下列標準進行過濾篩選1.對每一個串聯質譜譜圖的搜索結果列表，只提取排位在第一個的結果，其他的則丟棄；2.搜索得到的肽段中如果有KR，KK，RK，RR等序列，則這個肽段丟棄；3.搜索得到的肽段，長度在8個氨基酸以上的保留，小于8個氨基酸的丟棄；4.重復打到的肽段，只計算其中分值最高的，把這些肽段分值加起來，做為蛋白的得分；5.蛋白的得分超過60，就認為是可信的結果。低分的蛋白，手工檢查后保留或者丟棄。匹配到宿主或者牛胰酶、角蛋白的肽段，則丟棄。
實施例2日本血吸蟲表皮蛋白的生物信息學分析和功能注釋
1.同源性和相似性分析日本血吸蟲表皮蛋白序列對GenBank(http://www.ncbi.nlm.nih.gov/)的非冗余數據庫(Non-redundant，NR)做BLASTP比對；日本血吸蟲表皮蛋白序列對Swissprot數據庫做BLASTP同源性比對。根據比對結果得到的與已知其他物種或已知的血吸蟲蛋白的序列相似性，做出日本血吸蟲表皮蛋白的功能注釋。
2.文獻查檢根據已經發表的文獻報道，發現鑒定得到的23個表皮蛋白在蟲體上的位置，有一些的確在血吸蟲表皮上。而其他則是新發現的表皮蛋白。
舉例說明，比如以下表皮分離和質譜鑒定得到的蛋白，根據文獻報道，就是已知的表皮蛋白，而且有些蛋白已經有進行了疫苗和抗原研究。
paramyos intropomyosinmyosindynein light chain 122.6 kDa tegument membrane-associated antigentegumental antigen Sm20nitric oxide synthase 1(NOS1)leucine aminopeptidase14-3-3 proteinsSnaKcathepsin B-like cysteine protease precursor
21.7kDa antigenFK506-binding protein 50(immunophilin)實施例3日本血吸蟲表皮蛋白的實驗驗證為了進一步用實驗的方法驗證用蛋白質組學手段鑒定得到的血吸蟲表皮蛋白，選取了一個Immunophilin(SjFKBP50，GenBank登錄號為AY815389)做免疫熒光抗體原位雜交做蛋白定位分析。
實驗方法血吸蟲成蟲用冷凍包埋劑(OCT)完全包埋，切成5μm厚的切片。用抗Immunophilin的抗體覆蓋切片，在37℃反應60分鐘。該抗體是用重組表達的Immunophilin免疫小鼠制備的單克隆抗體。隨后切片用PBS洗滌，再加入FITC-偶聯的兔抗鼠免疫球蛋白的抗體(1∶40稀釋，用含有0.5mg/ml的Evans藍的PBS稀釋)，置濕盒中37℃反應60分鐘。染色結果用Leica DM-RB型熒光顯微鏡觀察并拍照。
圖-所示為免疫熒光抗體原位雜交實驗結果，表皮T；腸道G；下表皮ST。綠色熒光部分是Immunophilin的信號。
該結果證明了Immunophilin確實分布在血吸蟲成蟲的表皮上。這說明前面表皮分離和質譜鑒定的結果是可信的。
序列表<110>上海人類基因組研究中心<120>日本血吸蟲表皮抗原及侯選疫苗基因、蛋白及其應用<130>NP-10261<160>46<170>PatentIn version3.1<210>1<211>1462<212>DNA<213>Schistosoma japonicum<400>1aagttttcgc ttcactggat tatgataggt cgtcttcctg ccattgtgat tgataatggg60acagggtact ctaagatagg ttatgctgga aatacagaac ctcaatcaat catcccttcc 120tgcatagccg taaaagaata tagcaaggtt ggtgcaagag aagtcagacg acttgggact 180ggtttagatg acttaaattt ctatatagga gatgaagcta tagctttaga cggaaagaat 240gatttttctg taaagtggcc tattcgtcat ggtataattg aagattggga tttaatgcaa 300cgttacatgg aacagcttat cttcaaactt cttcgagcag agcctgaaga tcattatttt 360ctcctgacag aaccgccact gaatacaccg gaaaatcgtg aatacttggc tgaaatcatg 420tttgaaacat ttaatattcc tggtctctat attgctgtac aagctgtatt agcattatca 480gcatcatggt gttcaaatcg aactggtggt cgttcactta ctggaactgt tattgattcg 540ggtgatggtg tcacgcatgt gattcctgta gtcgatggtt atgttattgg atcatgtatt 600aaacatatac caatagctgg ccgggatatt acttcattca ttcaacatct gcttagagat 660cgcgagacaa atatcccacc ggaattaagt atggaggttg ctaagcaaat taaagaacgt 720tactcttatg tttgtcctga tttgcctaaa gatttgctaa atatgatagt gaaccagata 780aatggattaa accacatgtc gtttcaaaat ctgcgaaata tccagcattt tctgttgatg 840tcggttatga acgtttcttg ggtccggaaa tattttttca gcctgaattc gtcaatccag 900actttacaac ctcattgagt gatgttgtcg atgaagttat tcaaaagtct ccaattgatt 960ctcgccgtgg tctttatggt aatattgttt tatcaggagg cagttcaatg ttcaagcatc 1020ttgatcgtcg tttacaaaga gatattaaac gaaatgttga taatcgctta aaactaacag 1080aagaacttac tggcggacaa atgaagccta aatctataga cgtcaaggtg gtttcacatc 1140ctatgcaacg ttatgctgtt tggtttggtg gatcagtact tgctaatgag agtgaatttt 1200acaacgtatg tcataccaaa gcacaatatg aagaaattgg cccagctatc tgccgtcata 1260atccagtatt cggtacaatg acataattgt gatggagaaa ctttattatg gtttcctttt 1320tatattttct acatgatttc gcttcgttat tttatgatta atataatgtt ttcttactaa 1380actgttgaat tgcactaact caatctgctt actagcaatc ttatcgtttt gtttaatttt 1440taactaataa taagaatgtg aa 1462
<210>2<211>2000<212>DNA<213>Schistosoma japonicum<400>2tttaatcata gacttttaca catatttcga ttgccttagg tgctagtatt gttttgtctt 60atctcactag ctagaatgcc gagcagcaat gttgctaatg aagttatgtt gtcaggaaag 120ccagagaaat ttatagataa aatcgatcct gaggatccag attatcaaaa aatcctaatg 180cgtcctgctg ctataaaaga agatgttaat ttaatgattc agcgacagcg tgtttctgga 240atattgaagt cagatgcatt tcgtcgggaa ctggaagaag ttgttcgttc tcaatgtcag 300tctggtgact atcctggtat gagtacgagc cttctatctc tgcagcatat atcggacttg 360ttcacttccg ctccttctgg acagcctgcc agtgggggtg gattttcaag aggacatcga 420agtggtgtta tccctataaa tgacctgcgt ggtggtgacg ctactgttta ttctcgccgt 480gaacgttctt tgcgttgtaa gctggcttca gtatatcgcc taatagatct ctttggatgg 540aattctagta tatacaatca tgttactgta tgtgttagtc gcaatcatga gcattatctg 600gtgaatcctt tcggattgct ttatcatgaa attaccgctt cttctcttat caaagtcgat 660tctaaaggcc aaatacttga tccaggatct actattttgg gtgtaaacca agtgacctgg 720gctttacact ctgcagtaca ttcagccagg gctgatattc gttgcattat acatttggat 780accccttcaa ctgttgcaat atcttgcatg aaatcgggtt tattgcctct ttgtaatgaa 840gcaatgattt taggcgaagt ggcttattat acacctagta gtggattgag gtgcaaaact 900aatttcaata caaatggaga tttcagtgat gctaaagatt gggcagctga atgtgcaacg 960gtcagtgaag ctttaggtcc aactgctcgt atattgtttc tacgaagttt tgggctactc1020gctctgggtg aaactatcga ggaggcatgg cactatgcga ctaatgcaat agttgcatgt1080gatactcagc tcttattagc ctcagttggt gctgaaaatc tcatccttcc tacagacaaa1140ttaaagcaaa aaacgtatga aacatggcgt actgctgggt taggcggtct tagtggtcca1200gaagctgctc tggctatgag actatcttcg cctaataaat ctggcactag tgaaacagga1260caacatggag caacgaatgg atttcttaca gatggaggta caaatgtgga aactactttg1320tccagaccgt ggcggctggg tgagctcgag tttgaagcta tgatgcgtca cttagataat1380gctggatgtc gtacaggtca tctttatcga caggaggcta tggcaagccc acacagacct1440cgaaaaagta ggcccgttaa tggacacagt ggagctggta gtcaagatga agaaatacta1500gtcccaccat ctgcctcatc tgtaactgct acaatggctt actattatga cgatggagtt1560ctttcgggtg atgaaacaga aacacctagg ccgtcaactc ttaggcgaga gttttcagat1620aagcaatggt ttaatactcc aaacaaatat actaaagaag taattgaacc tgctccaggt1680catcaaccag tttcccattg ggtttgtgaa cgtgaggcgg ctgtttataa tgctgtttgc1740aatggtgccc tacctcctcc agcaccgttt catcctctta ctcatggggc agtaccacta1800ggttcaactg ggggacctgc tacagggact tcgtcaatta catcaccgta caatgtgttc1860gctccgcaag gttctaaccc caaggaatat cgtgaccaac agaaaaaagt caaggcaaaa1920tattaccatg atacaaatac tgccggacct caatctcgac tgttggacgg ccttacatgg1980gatgaagctc gacgagttcg2000<210>3<211>1129
<212>DNA<213>Schistosoma japonicum<400>3cgtgagtgta gagtcaactt gcggggattg gaggcgtgga cacaacgact ggcatagtag 60tgttggagat aatacctcaa tacaaaaaaa atcagaatca tgcccataca gtgcagatat120tatgaggact tattccctga agttggggat gttgtagttg taacagttaa ggtcatccaa180cctatgggta gctatgtgga acttctagag tacaagaata ttggaggtat gatattgcac240agtgagctgt ctcgtcgacg tataagatca atcagcaagc ttgttcgaat cgggtcaaat300actgaggtga ctgttgtacg agtcgatagt gccaaaggtt atattgattt atcaaaaaga360agagcttctg cagaggagat cgcaaagtgc aaggaaaggt ttgcaaaggc taaagcggtc420aatcaaattt tgaggaatgt agccgagaag ttagagtatg agacagatga acaactcgaa480gagctgtgca ggaaaactgc ttggtatttt gacaaaaaga ctggtcgaaa ggcagggtcc540tacgatattt tcaagaaggt tgtaaattca ccagaaattc tcgatgagtg tgacatagac600cagccaacaa aagaaatgct tcttacggat atcaggcatc gattaacacc aaaggcagtg660aaaattcgcg ctgactttga agtttcatgt ttcacttatg atggtattga tgccgtcaga720agcgcacttc gatctggact gaaactcaat tcagatgctc ttccaatccg catcaatttg780atagcgccac cgctttatgt actaaccacg caaacaatgg atcgagctgc cggcttagaa840cagctgaatg aagtgctaaa tgtcatccag acatccattg aaagtcaagg tggttctttc900aaaattcgac aggctcctcg tgttgtttca gatacagatg acgcagaatt acagcgtcaa960atggatgaac tagaaaaggc gaatcgtgaa gtttctggtg atgaggatga tgaagacgat 1020gacgaggatg aagatgatga tgaggaatcc aatgatggag atcagaatga acataagtaa 1080atatattata tgaatctaat aattaaatac atggatgaat agagttgcg 1129<210>4<211>457<212>DNA<213>Schistosoma japonicum<400>4cgagaataca ccaaccattt tctggggtgt agtggtgtgc tgttcgctta cgaataaaga 60ttactgatgt ctggtcgagg taagggtggt aaaggtctgg gcaagggtgg agctaaaaga120catcggaaag tgctacgtga taatattcag gggataacaa agccagctat tcgtcgcctt180gctcgtcgtg gaggtgtaaa acgcatttct ggtttaatct acgaagagac tcgtggtgtg240ttgaaagtat tccttgaaaa cgtaattcgt gacgcggtta cgtacacgga gcacgccaaa300cggaagacag tgacggccat ggatgttgtt tatgctttga aacgtcaggg tcgcaccctt360tacggttttg gtggttaggt tttggcaata ctttgaaacc attttattaa tcaactttaa420tgtacatagt gttttattta ataatatgta ttttctc 457<210>5<211>1695<212>DNA<213>Schistosoma japonicum
<400>5gtttctcacg tcccacatgt ctcttacttc gctttatact tgtcgggtga ttgtgaatta 60tcatccttgg tcagttgtct actcatgttc gatgcgatgt cggcattact ctaagggttt120catacagagt ctaattgaaa aagttaaaga ggaatcaagc aaagacactg agttaaaaga180aaatataaga aggtttcgag aggagacagc aaaactagaa cagacgaagg aactacgctc240cgtccgagaa ttttacaaaa agatagaaaa agaaatgccg gccgatacag ttgataaagt300acgaggcact tttcagtcgg ttaaaaacaa aattcgtaat gatgctcaaa atttggctca360aaacgaaaca ctaaggaaag gtgtagaaag tttatcaaaa gctggtgagc atgttagaga420tgccactaaa tcacttggcg aaaccgattt tgtcaaggcc gctataaaaa gtgtcgaagt480gctcgaaaaa gagttagaat cggataggtc taaaatgtat caagctccaa gtattttaaa540gacaagaagc gaaatttctg gcaaaagtga agatcgaata atgcagccgg acagtgaaag600ttctggcgtt gttgtacata aagattcgaa atggtttact gcatggcaag atttcaagga660aaataatccg tacgttcaaa ggttttttga cctaaaagct aattatgaag aaagcgatca720cttgatcgtt cgatcgttac gattcgtaac tgataaagtc tcaagtctat tcggtggctt780taaaatcaac aatgagctac aagaagtgct tgatgaagtc acaaaaatgg atccttcttt840taatttggag tcatttatac gttactgccg atttgttgtt atcccgaatg tacttgaagc900catagtcaga gtggacttgc caattcttca ggattggtgt cacgaagcgc ctttcaacgt960tctggccaca cctttaaggc agattaagga gcttggatat gtttctgaat ctcgcatact 1020ggatatacat aatgtcgatg ttcaaatggg taaaataatg gagcaaggac cagtattaat 1080cattacattt caagcccaac agatacattg tattcgtgat aagaatggga ctgtacacga 1140aggtgatcca aataaagtat tgcgtgtgac acatgtttgg gctttgtgtc gtgatcaaag 1200tgaaatgcac ccattagcag catggcgttt gttagacatc gccatgatac cctccgaaca 1260atgggtgtga tatttgttct ttactattac tctaaaaatt gtatatttca aaaataatta 1320ttaagtatct tgttaatttt taaacactaa taatgtatgt acagctttgt ttatttaaca 1380ttatacatag attagtgctt tgtttttttg attttttctg accagttagt acacgcacaa 1440ttatcaaggt tttgtgcata atttcctaat tcaggtaatt tgtcttctac ataaagcatt 1500ttttaagtat tgaactagat tttagtttgt atgatgaaca taataacaat gtattccgtc 1560ttttttttat tagacagttt aaaatcactt gctatcttct ttttttcttt taagcagtca 1620aagtacttga ttgtctactt attccaagtt gttttatgta catatttaaa tatctgttct 1680cattaaccaa aatct1695<210>6<211>1479<212>DNA<213>Schistosoma japonicum<400>6tcatttcagt taacttttat ttccttctaa attcggcaaa agaaagaatc taatcatttg 60tttaaatcat ggcatttatc aatctgctgt tgaggaatac aggaaggaac aatttcgtgc120gattttctcc cataaaccgt tcagtagcgc tgagtagatt tgaggaaaac acactggatt180atgataaatt ggaacacagt ctgagtactg ttaagaaacg actcgggcat ccgttaacac240tagccgaaaa agtattgtat tctcatttag ataatccaaa gactgcaaat attgaaagag300gtaaaagtta tctcatgctt agaccagatc gtgttgcgat gcaagatgct acggcacaaa360
tggcactatt gcaattcatt agcagtggtc taccacgtgt cgctgtacct tcaacagttc420actgtgacca tctaattgag gctaaggatg gttcagaaac tgacctcaat cgtgcaaatg480atgttaacaa ggaagtatat gactttcttg catctgcatc ggctaaatat gggatcggat540tttggaaacc tggatcagga ataattcatc agattgtatt agaaaattat gcatttcctg600gggcattaat catcggaact gatagtcata caccaaacgg tggaggactc ggtggtctgt660gtattggtgt aggtggtgca gatgcagtgg atgtaatggc taatttaccg tgggaattga720aagcaccatt agtaattggt gtacatttaa ctgggaaatt atctggatgg acaagtccaa780aggatattat attaaaggtt gctgaaattc ttacagtgaa aggtgggact ggagctatta840tcgaatatta tggtcctggt gttgaatcta tttcatgcac aggtatggct acgatttgta900atatgggcgc tgaaatcggt gccacgacgt caatctttcc atttaacgat cgaatgttgg960attatttacg tgctacaaat cgggcggata taggagattt agcttcaaag cacaaggctt 1020ctttacttac accagatgta aattgtaaat atgatagaat aattgaaatc aatttggata 1080ccttagaacc acatgttaat ggtccattta caccagactt ggcacatcca atttcaaaat 1140tatccagtca ttcatcaaaa gaaggttggc cacaatatat tagtgctggt cttattggtt 1200cctgcacaaa ctcttcatat gaagatatgt cacgtgctgc cagtttagct cgtcaagctt 1260tagataaggg agtaaaaata aagtcgcaat tctttataac acctggtagt gaacaaatca 1320gagcaactat tgaacgcgat ggtataacta aagtgtttca atctgttggc ggtatcgtgt 1380tagctaatgc ctgtggtcca tgtatcggcc aatggtttcg aaaggataca aagaaaggcg 1440ataagaatac aattgtctca tcgtacaatc gtaatttca 1479<210>7<211>678<212>DNA<213>Schistosoma japonicum<400>7agaaatgagt gcttcagctt tggtgatatt gtctgaggga gctgaagaaa tggaggcagt 60gacagttgct gatattcttg caagagcagg tgttaacgtt actatgggcg gccttcaagg120tgatgcgatt ataaagggaa gccatggtgt tctcataaag ccaaatgtgg cactttctag180tgtctcatca aaactgtttg acatggtagt gatgccaggt ggtatgggtg gttccaatgc240tatggcggag tccaaattag ttgggcaaat acttagagaa catgaaaaac acggaaaata300cattgcggca atttgcgcag caccgattgc cttggagtcc cacaaaatag cggtaggaaa360aaagctaaca tcctacccag gtttcgaaga caggcttcgg agttatacat attgtgaaga420tatagttgtt gttgatgata agcttgttac aagtcgaggc ccagggactg caatcgcttt480tgcaatgaag ttagttgaat tgctatgcag caaatcaaaa gcccaaacct tagcaaaagg540catgcttgtt tcgctctaaa aaacttacat tacctgtgtt ttatttcatc aaagtaggta600taataaacgt catctaacga atgaattttt tcgtttgtgc atcagtattt gctctagctt660tatcaaccta gcacactc 678<210>8<211>866<212>DNA<213>Schistosoma japonicum
<400>8cttgggcgtg acttaagtgg acgtgcctct gttgattgtc gattgtgttc atccattaga 60ctgggattat taccttatag gacttctcaa gatggatgtc gataaagcgc taatggaaaa120gctggagaaa ttaaagggta tgagtgacca agtacgtatc ggtggtaaag gaacggcccg180ccgaaagaaa aaggttgttc ataagaatgc tgctgctgat gataaaaaac ttcaaagttc240actaaagaaa ctaaacctta ataccattcc aaccatagaa gaggttaata tgtataaacc300agatggtaca attattcatt tcaagaatcc gaaagtccaa gcatccccac aagctaatgt360gtttgcagtt tctgggcaag ctgaatgcaa agcaataaat gacttgctcc caggtgtgct420taatcagttg gagtcggcaa aattacggct gtcaaaaatg aatgagaatt caaaaacatc480ggattcaaag atagatgacg gggatgatga tgtgccagat cttgtggaag attttgacga540gaaaagccgt ttggaataat cttaattatt gacaaatgta ttttgagtga atatgattga600tctagttact tttcaatgaa agtcattcaa ataccaacaa taatgtacaa tgtttggttt660ctaatgtctt attacactca gcttctatta ttcgttccct tttattactg actgtgcgtc720gtccaggcta tcctgctaat tttcattttg tttgacaaaa agtcttttac aaataaaata780aaactctgtt ttgtaccagc tagaatcaat ttgtatagct cactgcatta tcattaaaat840gaatggaatt ttgaaaaaaa aaaaaa 866<210>9<211>835<212>DNA<213>Schistosoma japonicum<400>9ttagatagtt gattgagtaa agcgtggttg tctacatgga ttttgaacag gagataaagc 60ttttcggaaa gtggagtgtt gatgacttaa atgtaaacga tctcagttta acggactata120taaacatcaa acgcaaagga aatccgatcg tacctcatac agctggtcgc tatcaagtta180aaagatttcg caaagcacta tgtcctttgg tggagcgcgt ttgctgctcg cttatgatgc240atggacggaa taatggaaaa aaagttaagg caatgaatat tctgaaacat acttttgaga300tcatacatct actttctggc gagaacccaa ttcatgtgct agttaacgca gtactaaaca360gtggtgctcg ggaagactca actcgtattg gccgtggtgg gactgttcgg cgccaagctg420tcgatgtttc tcctttacgt agagtgaatc aggcaattta tttgttatgt actggcgctc480gcgaagcagc atttaggaac gtaaagacta tctcggaatg tctggctgat gaacttctga540atgcggctaa gggaagttca aattcatatg caatcaaaaa gaaagatgaa ctggaacgtg600ttgctaagtc taaccggtaa tgtaaagctt agaataaaac ttccggagtt gaaaaaaaaa660aaaaaaaaaa aactcgagtt tttttttttt tttttttttt ttttaaaatg agagattggt720tgttcaccaa agaaacaaaa cggattttta tggaagaaat cacaatatgt aaacgtaaaa780ttagcatact ataaatcgca taattgtccg gttatttgaa cttgacgatt acaaa 835<210>10<211>717<212>DNA<213>Schistosoma japonicum
<400>10tcttagcgcg tttttcgacg gttgttttgt gattatggtt tttcgtagct cgatgagttt 60ggtgcacatc ctgaggtcta gtgttcttcg caattatgga gtacgcttta aacatacatt 120accacctttg ccatacgatc caagtgcctt agaacccgtt atatctaagg aaattatgca 180attgcatcac tctaaacatc atgctgcata tgttaacaat ctcaatatcg cagaggaaca 240gtttgctgat gccatgtcga aaagtgatgt tacaaaaatg atatcgcttc aaccagcact 300tcgttttaat ggtggtggac acataaatca tagcattttt tggcataacc ttagttctaa 360gggcggcggc gtccctactg gcagtctcgc taatgctatt aatgatgagt tcggatcatt 420cgataacttc aagtctagac tatccgctac cacaattgct atccagggtt caggttgggg 480ttggcttgga tacaatccca acacaaagcg tctacaaatt gccacatgcg ctaatcaaga 540ccctcttgaa gggaccacag gtttgaagcc tttacttggg attgatgttt gggaacatgc 600atattatctc cagtataaga atgcacgtcc agactatgtg aaagcgattt gggatattgt 660aaattggagt gatgtagcta agcgatttga cctatgcaga aattaatttc accatgc717<210>11<211>1431<212>DNA<213>Schistosoma japonicum<400>11tgatggctga atctgataag ttaaacattg acagtttgat atctaggctt ttggaagtcc 60gtgggtctcg tccgggaaag aatgttcaac tatcagaagg agaaatacga gggttagctc 120tgaagtctag ggaaattttc cttcgacagc ccattctgct tgagctggaa gcaccgttga 180agatttgcgg tgatattcat ggacagtatt atgatctcct tcgtttgttt gagtatggag 240gcttcccacc agatgcaaat tatttatttc tgggggacta cgttgatcgt ggaaaacagt 300ccctcgaaac catttgtctt ttacttgctt acaaaataaa gtaccctgaa aacttttttc 360ttctgcgtgg aaatcatgaa tgtgcatcga ttaatcgtat atatggcttt tatgatgagt 420gcaaacgtag atacaacata aagttatgga aaacgtttac tgattgcttc aactgcctgc 480caatagtcgc cattgtggat gaaaagatct tctgctgcca tggtggcctc tctcctgacc 540tccattcaat ggaacaaatc cgtaggatta tgcgccctac cgatgtccca gaacaaggtt 600tactatgtga ccttctttgg tccgatccag ataaagatat tactggctgg ggagagaatg 660accgcggtgt aagctatacg ttcggcgcag atattgttac caaatttctt catagccatg 720agctggatct catctgcaga gcacatcaag ttgtagagga tggctatgaa tttttcgcca 780aacgccagct tgtgacctta ttttctgctc cgaactattg tggagagttt gataatgctg 840gggctatgat gtctgttgat gaaactctta cttgttcatt tcagatatta aaacctgcag 900ataagaaaaa attcaacttt cggtctctca attctggatg gcctaccact cctcctagaa 960attctaaact cgaagacaaa tgagttgttg ctatatctca tcgtaagtta tcagtttcct1020gaggccatgg agactacatg aaccagtaat ttgattatgt acagttgtta aaaacttggc1080aacctaatgt atcatttatg ttagttaatc acttcactgt tatgactaaa ctcttacaat1140ctgctgtttt gtttcatgtt tatctcgact ttcaatttct aaaatctttt tctttcgtct1200ctgtttaatt tcactagaga taaaataatg tacagacctc tgcccatgtt tgttatcgac1260ttatcattgt ataataatct ccatctgttg tatttcataa cagtaggcgc catgttttca1320ttgtaattac tgcatttcac catcgacgag ccttttatgc ttatgcacgc tacagaagtt1380
tctactcaac ttattaagac gcctgtataa ataaagatta taaaatcttt c1431<210>12<211>922<212>DNA<213>Schistosoma japonicum<400>12tccgagtgga gcagtgtttt gattcttctc gtgatctgtc tgcacgtttg tcagcaatca 60tgtctggtgg ctatccagct ttagaactgt cagcagaaga cctgcgttta atgcttgctg120cccatgttca tgtaggagaa acgaatatga atttccagat gtcgacttac gtccatgggc180gtacaaaaga caattttcac ataattaagt tggacaaaac atgggagaaa attctcctat240cagcccgtgc catcgcagcg attgatactg catcagatgt ttttgcgatt ggatctagac300cattttctca gcgtgccgtg ctgaaattcg ccaactacac tggtgcaact gcaatggctg360gtcgcttcac acctggagca ttcacgaatc agaaacagtc ttgttttcgc gaacctcgcc420ttctggttgt cagtgatcca cttagtgatc atcaagcagt gatggaagct agttccgtaa480atgttccatt aatcgcacta tgtaataccg actctccatt gactcgtgtt gatattgtca540ttccctgtaa taataagtca actcattcta ttgctgttgt ttggtggttt cttgctcgtg600aggttcgacg catacgtggt gaagatacca gattgcaaca gtggagcgtt ttacctgatc660ttttccttta tcgcgatcca aatgaggaag aacaaaatgt tgaggaccct gaagatgcca720gattagagcc agttatacca ggggccgatg ctgaagctgc agagacatgg ggagtggaac780catcaatgcc tgttggatcg cttggtgata tggggattgc tgcccctggt tacggtcctg840ttggtgggac tgccggtggt tggagtaatt tggatgcaga tccaacagaa acatggtagt900ctgctaatat aacttactat gg 922<210>13<211>1058<212>DNA<213>Schistosoma japonicum<400>13gaagttctgt gtcaggaaaa tccagataaa tcagaagctg tagttgcaat cgaagttctt 60gcaaaacttc atgaatgttc cgatgttacg acggttcaag gtctttacga tgtaatgaat120gcaaccatag aacgtatgtg gaaagaggga gttagcaatc tctctgttat atcagcctgc180gaattatttc agcgttttat tacgttgaca acactggaca ccgttagttt tgatgagtgc240aaaagagttc tttcagagag ggcccaaata ttcattagaa aggtcggttc gtgtagacgg300aaaatcgcag aaaacttttt aaattacatc cctaatggtt cagtaatatt cttacattct360tactctcgag ttgttcttgc tgcactagca tatgctgcat ctgctcataa acgtcttcat420tgttatgtga caacatgctc acctaacggt ttgggagcga aaatgacaaa agccttagct480aaacttaaaa tttcctgtac acttgttcca gacaattcac ttgcctattt gatgccgcaa540gttgattttg ttgttctagg tgctgaagct gtagtggaaa gtggtggaat attaaatata600cttggctcct cactaattgc aatgacggca tcctcattgg gtaaaccagt atatgtgtta660gctgagtctt tcaaatttat gcggtgttat cctcttgacc aacggcatat acctgatgaa720
ttcaaatggt catctgatca tgaatataaa tcattatcat caccatcacc tctgtttaat 780ttaagagata aaagcaactg ttactacttc ccaaatgatg atgatgacga tgagaataaa 840tgcgtcttcc cagaaatgaa aacagtatgg gctgctgttg aagcacagag agtttctgta 900attgaacaaa agatgcctcg agtcgactat acaagtccat gttacataaa ttatcttata 960acagatttag gtgttttgac accttcagct gttagtgatg aattaattaa attgtattta1020taataattgc aaccgtaaaa atatactcca atttatac1058<210>14<211>1855<212>DNA<213>Schistosoma japonicum<400>14tctgtggtac ttgttaaagc gccgtttaaa actctggaaa ctactcgctt ctgttaacaa 60agtttacttt catctgtttc gggcccgata gctgccaacc atatagtgaa tgacatctca 120acaagcctcg gattcatcta gttgtgaatt cagtccgtgt tcagaacaag ttgtgaactg 180aagtagacac tcaaattcgt tgttatggat acagttaggc aagccgtaac gcgcaccgct 240cttggactag cagagtatgt gacacctgtg ttaaaagcta ctaaattccg agagacagga 300gtcatcactc cagaagagtt tgtagccgca ggggactttt tagtttatca ttgtccaact 360tggcagtggt caatcggtga taagcctgct cgtccctatc ttccaaaaga aaaacagtac 420ttggttacta gaagtgtacc gtgctacaaa agagtaaagc aaatggcaga ccatcacgaa 480gagtttgaga aagtattgga ggaggaggat ggcgatggag gttgggtaga tacacatcat 540tatgcaacca aatatggtga ccttaaccct gaggttgaaa aaacgaatga aatgtctgtc 600ccttctgttt cacattcgct caaaaaatct aacctcgaag aaaccataat ttctgattgt 660gaggatgaag aagatgaaga catggaagct ttcctacaga gtggcatgct ggatgagtgt 720gatcctgcag ctgttaaagc ccgaacaaaa gtagccaatg atggaaatac gagccaagct 780atggcagact ttggcataga ccaaaatata gatcgtgaaa ataatggaat tcttcaaaca 840cggacctatg acttgtatat cacctatgac aaatattatc aaacaccacg tctatggttg 900tttggttatg atgaacaaca tcaaccattg acagaaactc agatgtacga agacttcagt 960caagatcatg cgaaaaagac tgtcacgact gaagcgcatc cacatttatc cggtcatatg1020atgccgtcaa tccatccatg tcgacaagct gatgtaatgc gaaagttaat tgaggctgta1080gcggataatg gcgctgaatt agctgttcat cagtatctta tggtattcct aaaatttgtt1140caagctgtta tacctacaat tgagtatgat tatactcgga atttcaacct ctcttcatat1200gcctaatatc cactctctgc atagtatatg tactaaatat tttattgact aaactgttat1260agttcggact tactaaaatg tctttgagtt ccagatgtct attttgagag tctatacaaa1320ttcctaaatc acttactttc aagtattttt gaaattgact gttggtttcg gtagtaaata1380catgttactt tgagaaaaca cttttctgat ttcatgtagg attccccata cgtctacgta1440tattgcgatc ttatctgttt tcgtaatcac ttataagcat gccttttctc ggtaatacga1500gttaatgagt ttattctttt ctaaattgtg ttgaccaacc cgttttttca gttgttgacc1560ggataccagc caaacttaac atcctacaat tttgtactgc tcctaaagaa tgaaaggtgg1620tattcacccg ttgttaatat tttcacgttc gtatttccct attgtaatac ggaatccaaa1680gagaatgcct aaaacaacgc aattcctata tctcggacta ttcatgcttc caatggttgg1740tgcacttggg atatgcacaa ctttcaaatc attaccgacg cagtacaaag gtccagaact1800agtgaagtaa tgtaaaactc ttcagttagt tgaatatagt gtagaaatga aaaaa 1855
<210>15<211>917<212>DNA<213>Schistosoma japonicum<400>15cgtcatgagt tccttagtcg ttgatagggg gcccggtggc ttttcatttg ataactgcta 60cagaaacaaa gtaattgaac agaagggttt taaagtaccg aaagcagtaa agactggaac120tacaatttgt ggagttgttt tcaagaatgg catagttcta ggcgcagaca cacgggcaac180agagggcaac atagttgctg aaaagaattg ttctaaaatt caccgcattg cagataacat240atattgctgt ggagcgggga cggcagctga cactcaaatg gtcacattga tgatttcttc300ccaagtcgaa cttcaccgtt taaatacggg tagaactcca cgagttatag ctccacttcg360tcttcttaaa cgttatcttt atcaatatca aggttatgtc ggtgctgcac ttgtactagg420tggtgtggac tcaacaggtc cccatttgtt tagcattgca ccacacggat caacagataa480gttgccgtac atcaccatgg gtagcggtag ccttgcttgc atgtcggtgc ttgaatcaag540atttaaattc aacatggaac aagatgaagc cgtaaaatta gtacgtgatg ggatagcagc600tggaatattc aacgatatgg gatctggcag taatgttgat atatgtatta tcactaaaga660tggcacaaaa tatatccggt catatgatga agctaatgtg aaggggaaaa gagcagagaa720gtacaatcct ccagaaggaa ctacttctgt tcttaacaaa agtgttcatc gtgtggagtt780cgatgtagtc actacacgcg ttgttcgaga tatgcctgtt catgatgttg aaacaatgga840tctttcctaa gtgtatatct gagtttttaa atgaaatgcg cttcatacat tgttctaata900aagatgtctt tttgttt 917<210>16<211>1477<212>DNA<213>Schistosoma japonicum<400>16ttgtactcgg cgcattactt tgagatgttt tcgagaggtg tcaatgtttt caagttcggt 60attactttta gaagctttgt gacctcacca aagcattctc ttaaggttgc ggtactaggt120gccagtggtg gtatcggtca gccattgtca ctactcctaa aacagtcacc tctgatttat180cagcttgccc tgtatgatat cgctcatgta aaaggtgttg ctgcggatct tagccacatt240gaaactcaag ctcatgttac tccgcatctt ggaccaggag aacttggaga atgtctgtca300ggtgctaatc ttgtcatgat tcctgcaggg atgcctcgta aacctggaat gacacgtgat360gatttgttta atacaaatgc atccattgtt gcagaactta tcaatgcctg tgctaaaaat420tgtcctaagg caatgatatg tataatcaca aaccctgtca atagtactgt gccaattgca480gctgagattt taaaacgcca caatgtttat gatcctaaac gtctttttgg agttacaact540cttgatgtcg tacgatcgaa tacattcatt gctcaagcaa aagatcttgc tgtaaggaaa600gtttcttgtc ccgtcatagg tggccactca ggtattacga ttctccccgt gatatctcaa660tgtagtcccc atgtatcatt cccacaggac gaacgggaga aaattactaa acgtattcag720gaggctggca ctgaagttgt tgaggcgaag gctggagcgg gatctgccac cttatcaatg780
gcttatgctg gagcaagatt cgctatctca cttttagaag ctatgaatgg tcgggcagga840gttgtagagt gtgcttttgt gcagtcagac gttactgagt gtgaattctt ctcaactcct900ctcgcacttg gtactgaagg tgttgaaaag aatatgggta ttggaaaact aaatgaatat960gaaattgaat tactgaagaa gctcattcca gagctacaag cgaatataaa aaaaggcaaa 1020gagtttgcgg ctaaatttac acccaaatga aaatggatca tgattatgtt ttctctgtca 1080agtaattcgc ttagttattc tcgatacagc ttacatcctt atgtttctat tttcagtaat 1140ctttctttca tttttcacgg tatctggagt gatacattct tggggaaaaa attgtctacc 1200ctatcggtgt ttgggcctta gtgtttcttg attttccact ttgtcaaata tggaagtgtt 1260ccctattttc attttatctt tctccttcgt ttttccttca tgcatcccct tttttatcat 1320ctgtttcctt cattcccact aattttccag ttttcaatag tgttatgctg tctcagtgtg 1380cgtgtttgtg ttcatgaaca gtatttagtt aaagtcacta ggtttattta tattaatgta 1440cacttgaaaa aaataatata catttaattt cccggtt1477<210>17<211>946<212>DNA<213>Schistosoma japonicum<400>17gagggtagag gctgcggtac aaaaaatatg gctggaagta agtggccggc ttcagtggct 60cctactagca gagttggaaa taccccccga gcaaaacaag ttatttttga acctacattt120gccaagatgg agaaagaaaa ttactcttta actcagcgat taaaggaagc tttttataaa180gttgaaaaga gatatccagg cttcagtaaa gacttcatgg tcggcttgct tcaacgcatg240ggagttgata gtgaaattga tgtgacagaa acgtgtttac gaattgctgc tcttcaagaa300tgtgataatc caaggacgtc gagaaatccc agagttttgg agttagaact tacggccaaa360acactaaaaa ctattcttag tagtatacct gatgaaatta cagatcgcag agcatttata420gaagtcataa aaggtatagc tgatgctata aagatgttgt tagccgctgt cggtgctttc480tacgatgcac taccaccagg aacacaaaaa aagtgtcttg aagatcaaaa acgtaaattc540attacctact gccgtaaatt cagtgatact ttgaagcagt actttaggaa caacgatcaa600accgtagttt tcagcagcgc aaacctatta gttaaccaaa ccaatctaat tctctttgtg660gttcacgatg tggattgaag aaaagaaaaa tataattaat gatattcaac attttacttc720ctagtatatt tgcctataaa tgcaattcat gaataattga aaaatcaatt tgtgcttttg780taaaaacatc ctttgttaga cttgaagtga ttgctttttt ttaaacatta cgatagtgga840tttttgtgtt ttccaaatat ttgtctaact ggcaaatctc atatcatcac ttacgttttc900ttcacttcca gttctttgta aattaagaat agaagcttct aatgat 946<210>18<211>1608<212>DNA<213>Schistosoma japonicum<400>18tgaagtccga agattacact ctatagaact tattggtggt agttcacgta ttcctatgtt 60
aaaggacgcc gtcgtatcta tttttaaaca ggaaggaata acaactctta acgctgatga120agccgtagct cgtgggtgtg catttcaagc agctatctgc tcaccagctt tcaaagtgag180ggatttcaat gttgtcgaag catgtttata tcctattgaa ataaaatttg atcgagagga240aggcagtgaa caatgcatgc aaacagaaga gataggtggt gcaataacaa cgcagggaaa300agctatgcac atcgaggttt tccctttcct gcatcctatt ccttcttcgc gtcagatatt360tcttactcgt cgtggtgctc tcactttaga ggctcggtac gctaacacag aaggcttgcc420aaatggaaat gtagttattg ggactttcag gataggggag gcttctcaaa tgtttacgga480gcctcgtaaa atcagactca agttgcgtat gaacactcat ggtatcttca atatttcaca540agcacaatta gtagaagaat atgaaaagga agttgaagtt agtgtttccg aggacaaccc600tgtagttgat ggacccgcaa attcaacaaa ggaaatgact acagctgcta atgaaggtaa660cgaaaatcca aatccatcag cagacaatgg tcaagtaaac acacaagact ccacttcacc720aaaaaagaaa acagtattaa gaaaaaagaa attcactaaa taccatgact tgagtattga780agttaacaat atgcaattca gcacaaaaca actcaacgag ttttgtgaga tttgcgctaa840tctcatattc aaagacaaac tggaacgcga acgtgtgaat gccaaaaatg cagttgaaga900gtatgtttat gaaatgcggt cgaaactcca aggttcatta aatccatttg catcacccac960tgagtctgaa tcattacttc aactactaga tgcgacagag gagtggttat atggtgacgg 1020tgaatgttca aaacgtcaag tctatgtcga gaagctcgct gagttgaaaa acaagggaga 1080tccgattgtt catcgagcga atgaacatct aaaccgtcct ttagcagttg agaattttaa 1140ccgatcactt gtgcgtataa ggaaagtgat agatagcatt gcagcaggag aaccaactta 1200tgatcactta acacaggaac aagttaatca gttggagaat atgatagttc agtacgagac 1260gtggctgaat caacaaatga tgcaccaaaa tcctcgacca caacatgcag accctgttgt 1320taaagttaca gatattatat ctcaacaaca agccatggaa tcagtttgtc atccaattat 1380aaatattccc aaaccgacac caacgtctca aacaaataca gcagatccta actcccactc 1440ttctgagcaa tgcaacaatg tgaataatgc aaacacagac tgcaacacca aatcaaatca 1500ttccaaaaag tgtggcgata atgcagaaaa tatggactta gattaacgac cgcatgtgac 1560ttatatctct ggaatcttat gtttcattca gcattttatt caattctt1608<210>19<211>645<212>DNA<213>Schistosoma japonicum<400>19cggcgcgatc aattaaggca tggctccgaa agctcaacca aagaaacagg aaaaaagtac 60aaatgatccg gcaaaaaaga ctcaagatag taaaaaagga tccaagaagt ctcagaaaac120gtctaagtcg ctaaaagcct tgaaaagcgt aaagggcggt aaagtggtga aggctagtag180taaaaagtct gcagctttga aggcgttgaa agctcagcgg gctatcacac ttggttcaca240cacgaaggga aagaagagag tcatgtatac cacacgattt aggcgaccta agacgtataa300acctgcacgc tgtcctaagg atagaaggtt tctgatcccc aaaaggacca aacttgatgc360ctttcgcatt attcaacatc cggttacaac tgaggctgca atgaagaaaa ttgaggacaa420cgatacactg gtgtttattg ttgatcgtcg agcaaataaa ccggcaatca aagcagctgt480tcaggaactt tataacatta aagtggcgaa agtcaacacg cttaattgcc ctaacaatgt540taagaaagca tacgtgaagc ttcctcccga tttcgatgca ctggatgttg ctaacagaat600cggtattata taaattgtaa ataaattaat gtgctggttc ctaag645
<210>20<211>1227<212>DNA<213>Schistosoma japonicum<400>20attatcctgt tcaaaatggt gaaacaccta agcttttata tccacatgtc aatttcttgg 60gtatacctgt taacaaaggg caaccaaaac ttggtacata tcagggacca gattttatta120gaaaatctaa cttcttccag cttgtagctg aagatggaat ccaaataacc gactgtggag180atgtcatacc tgtagaacta agtgaatcag aggatccaga acgttgtgga atgaaatggt240caagaagttt cacacagacc actttgaaaa tagctgaccg tgtagaacag ttggtaaaag300ggtcaaataa acatagtatt gaatccagta attcgaaacc atcaccatta gtaattgttg360gcggtgatca tagtatggcg actggaacta tacttggaca tgctagagcc aaaccagatg420tgtgcattat atgggttgat gctcatggtg atataaatac accaccaaac tcaactactg480gaaatataca tggaatgcca ttaagttttc tagtaaaaga actacaagat caaattccat540ggttggatga ctttcatagt ataaaaccat gtctggatgc cagcaatctt gtttacattg600gtttacggga tttagacgtt tatgaaacac gggatataag aaagcatggt atagcttatt660ttacaatgct tgacgttgat cgaatgggaa tggaagccgt cattaaagaa gcattacaag720ctgtgaatcc gagattagag aaacctattc atttaagttt tgatattgat gcattggatc780cttcaattgc tccaagtact ggtactgctg ttccaggtgg tttaacatta cgtgaaggtt840taagaatatg tgaagaaatt tcagctacag gaaaactttc tattgttgaa ttggctgaat900taaatccttt gttaggatct aaagaagatg ttgaaaaaac gcaatcatct gctgtgcaca960ttttaagggc atcgttagga cattgtcgtt caggtcaatt accgatgaaa gttaacaatt 1020caaccactaa tagtattgtt agacaagctg aacgtataca gataaagtga taattattct 1080ttcttcaata gcaattaatt gatttaattc ttataataat ataattcaat gatcaatatg 1140attaattaat aatgttacta acaaaataat atgtaataat acaatgattt aagtattttt 1200ctaaatatac tactattatt atatttg 1227<210>21<211>1502<212>DNA<213>Schistosoma japonicum<400>21cttatcttca ttgtttgtga ctgttaacta aagaactgtc gaaattcttt taatgcctac 60caaatctcca gtctttactt atgtcattaa gagattaagt tctcactgga accatgttaa120gctgggacct ccagatgcaa ttttgggaat tactgaagcc tacaatcgtg acaccaatcc180tcagaaaatt aatctaggag ctggagccta tagggatgat aatggaaaac cttttgtctt240gccatctgtc aaagaggcgg agtcactttt attagcgaaa aatttgaata aggagtatgc300tccaatcagt ggcattcccc aattttgcga tttatccatt aagcttgcac ttactgaaca360gtctccacga atcaaagatc gttgcaatgc tacagttcag acaatatctg gcactggtgc420tcttagaatt ggtggagctt ttattaatga attcgcagaa caaaaacaca tttggatgcc480
gtcacctact tggggcaatc atatacccat tttcgtgcat agtggattga atgtacatca 540atatcgttat tatgatagta atacatgtgg atttgatatt gatggatgtt tgtcggattt 600gtcaaaaatt cctaaaggac attttgtttt attacatgca tgtgcacata atcctacagg 660agttgatcca tcatttgatc aatggaggaa aattggagag attatgaaat cacgtgattt 720aattccattt tttgattgtg cctatcaagg attcgcttca ggtgacatta ataatgatgc 780gaaagcaata cgttatttta cagacgaatt gaatttccct actttattcc ttacacaaag 840tttcgctaaa aatatgggat tatacgggga acgtgttggt gcgtttagtc ttctttgttc 900ttcatcagat gaagccgaac gttgtttatc acaaattaaa atacttattc gaccaatgta 960tagtaatcca ccaattcacg gtgcacgtat tgctgctgaa ttaatgtcta atgttgattt 1020acgtcgaaaa tggctcattg atttgaaatc tatggctgat cgtattataa caatgcgcca 1080aagtttaaag aatggtttaa tcgaagcagg atctcaacat gactggtcac atattactaa 1140tcaaattgga atgttctgtt tttctgggct taatccatca caagttgaaa aattaactaa 1200tgaatattct atttatttga ctaaagatgg acgtattagt attgctggat tgtcgtcgaa 1260aaatgtgaaa tatttagctc atgctattca tcaagttact aaataaataa gtaatactac 1320ttttactaat agtagtatta tgatgtattc aatgaaatcg gtgctttaca ttctttaatc 1380ttttgttaat ttatacaaat ttatttgaga atattgcaat gttcaacgcc tttgtcttga 1440ttcattcctg tgttttgtgc aagaatttct attactacgc aaatatattt ttgattgatt 1500gt1502<210>22<211>1559<212>DNA<213>Schistosoma japonicum<400>22tcggttaaca gaagagctta gatcgattcg ggaccaaact gcctctgaat tagaagagta 60caaaatacaa atggaagaaa cattcgagaa caaactagga cagttgaaat cgtcagcgaa 120tttcagtgct gaggatgctt gtcgtcaaag atccgagcta ttaatggctc gcaaacgtgc 180tgatgacctg tctcacgact tatccaagaa gattgccgaa ttggaattgc tccagcgacg 240agtaacagac cttgagggac aattgagtga tgaacgaaga gattttgaag cacagcttct 300tttccaacgt caggaagtag agagactaaa agaggaattg gaagaaagtt ttcgtgaatt 360tactgacctc atgaacacaa agatcgcact agatcaggaa atactaatgt atcgaaaaat 420gctagagggt gaagaatcta gactaaattt gacaccagct tctcgtgact cgccattcaa 480tatacacaca gttaagcgca ggcgtattga tggtgattac gatggtgatg actcgactgt 540caacttaact ggatcaagtt acagttcatc acgatccaga ttcgcctacc gagtttcttc 600tactgcacgc ggacctgtag aatttttcaa ggaacaagac attcatggca agtggatcaa 660aatatataac tcttcaacag aagaagttaa tttaggtagc tgggaacttg ttcatgaagc 720cgacggacac gagacccgtt tcaaattttc tcggactttc tctttgaaac cgggcacaac 780gtgcacgatt tggagccaag atgctgaagg caattcacat aatcc tcctt ctgatataac 840tatgaagaat aaaagtttcc atcctggtac tgaagtcaca atcttattac ttgacgcaga 900tggtgaagaa caaaccaaat gcacagtgaa gcgagagaaa gttcgtcctt ctgtggttaa 960cttcgatcgt aaatccggtg ttcgtggtca tgatgaaaaa tgtcatctca tgtgaaaatc 1020acgttttgga aggaagataa cctacattgt caagtttctt attgttttgt cttattatgt 1080taattcttcc gttaatatca agtttgtaat tgcattttac tagttttcgt ttgcttatgt 1140
catttgtagc taattagcta gcaaaagaat tcagattgtt tctgtctgta tgcgctatct1200tgcttgccca ttcatcgtgc ctattttaat gtaattcctt taattgacct ctggtcatac1260cctgtcattt tgccataata atttattttc aagtgttatt gcctcttttt aaacagtaaa1320agacgcatga tttttgtcgt ttatcacgtc atcattgtat cattgttatc gttacctcat1380ctgtatacta ctatccagta ggctaactat tgaaattcag tgtgcatgta tttgtctgcg1440tagatatata tgaacaaaat cagctataaa cactgctgtc ctgcttcacg cttatcgtct1500tcataaacac ctatataatt attaaccacg tttgtctttc atatgtattt aatacatat 1559<210>23<211>1781<212>DNA<213>Schistosoma japonicum<400>23atactgttag ctcacagcgc aactttcctt gattgacgag atggggcggc gcaactctga 60aaataatgac cgtcgtactg aacaattccg caaattattt ataggaggcc tgactcctct 120tacaacggaa gccaagctaa aagatttcta ttctcagtgg ggtgaaattg tagatgtcgt 180agtaatgaaa gatggtcgta ccaaccgttc caggggcttc ggcttcataa cctataagga 240accggagatg gtggatgctg ctcaagccaa ccggcctcac gaaatcgatg gcaagacagt 300tgaagccaaa cgagctatgc cacgtgagga ttctaacagc cccgagtctc atatgacggt 360gacaaagctg ttcgtgggtg gtttgaaaaa ggatgtcact catgaagaac tacgcgaata 420cttcagcaag tatggaaata taactgagtg tgaggtagtt gcctggaaag agtcgggcga 480gtctcgtggt tttggcttcg tcacatttga tgactacgat cccgtcgaca aagctatttt 540atacaagcca caccatatag gctccagtag ggcggatgtc aagaaagccc tcagcaaaga 600gcaaatcaac acaatgaaga agaagcaaga gtcgcggatc gactacaaca acaattatgg 660cggtggtggc tacggcccaa tgggtggagg ctacggcgga tatgaagggg gttacggagg 720ttcctatgga ggtggtcctt acggaggcta cggctcaaat ggtggaggta gcggcggtgc 780ctatggaggt ggcaattacg gaggaggctg gggtccaaat cacatgaacg gagatggccc 840aggctggggg cagggaggaa tgggcgatgg attcggacac tacggaaacc agcagaatta 900tgccggtggc ccgatgcgtg gtggtccacc aggtggttac cagcgaaatg ctccctatgg 960agggggctat ggtcggcgtt agtcacacag ggttagtatg gcctcaacag tttggtctag1020gcagttctac tagcaggaga gacagagttg tcctagtgcc ctcaagtcct aggtcagcca1080gcagaaagtc gacagcctgt ctatagctga ggaggccagg gatcctatta ttcactgtgt1140atatactatt aactgttaat tttcactgca tgtgcaatcg agcgttcttt ttaactgcca1200tattcattgc gagttagcat ccagtcgaga gtaacattca tctttaagtg taaagtttcg1260tatcataaat gcataagctc atgcagtatt ttttaatcca gctttgtcac tagtattgaa1320ggaaataaaa gtgttgaagc cagttagtct tttgagttga gttttatgat tggtagtaat1380gagggaaatg gacgcttgtt gggcagaaga caaagttggg taggtcttcc agccccatat1440gctgagtagc cacgtcagcg cgtcatgccc attaagtaga taaactgtca gtactagttt1500cgtcagagtt agagtaactt ccgttttttt gggtacttag agcaggtttg gggaacggta1560tccattcact ttgttcacaa tttttgagtg gttttccaga tatcggggac cctttggaga1620attaatttcc gtccgtggcg tggagcgcga tggactggcg aactcgtgtt tgtatgactg1680ggttgtcact ccatgctgtg atataatgtc aaaaataaat tcgcggtgtt actccacatg1740gcaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a1781
<210>24<211>305<212>PRT<213>Schistosoma japonicum<400>24KFSLHW IMIGRL PAIVID NGTGYS KIGYAG NTEPQS IIPSCI AVKEYS KVGARE VRRLGT 60GLDDLN FYIGDE AIALDG KNDFSV KWPIRH GIIEDW DLMQRY MEQLIF KLLRAE PEDHYF 120LLTEPP LNTPEN REYLAE IMFETF NIPGLY IAVQAV LALSAS WCSNRT GGRSLT GTVIDS 180GDGVTH VIPVVD GYVIGS CIKHIP IAGRDI TSFIQH LLRDRE TNIPPE LSMEVA KQIKER 240YSYVCP DLPKDL LNMIVN QINGLN HMSFQN LRNIQH FLLMSV MNVSWV RKYFFS LNSSIQ 300TLQPH 305<210>25<211>641<212>PRT<213>Schistosoma japonicum<400>25MPSSNV ANEVML SGKPEK FIDKID PEDPDY QKILMR PAAIKE DVNLMI QRQRVS GILKSD 60AFRREL EEVVRS QCQSGD YPGMST SLLSLQ HISDLF TSAPSG QPASGG GFSRGH RSGVIP 120INDLRG GDATVY SRRERS LRCKLA SVYRLI DLFGWN SSIYNH VTVCVS RNHEHY LVNPFG 180LLYHEI TASSLI KVDSKG QILDPG STILGV NQVTWA LHSAVH SARADI RCIIHL DTPSTV 240AISCMK SGLLPL CNEAMI LGEVAY YTPSSG LRCKTN FNTNGD FSDAKD WAAECA TVSEAL 300GPTARI LFLRSF GLLALG ETIEEA WHYATN AIVACD TQLLLA SVGAEN LILPTD KLKQKT 360YETWRT AGLGGL SGPEAA LAMRLS SPNKSG TSETGQ HGATNG FLTDGG TNVETT LSRPWR 420LGELEF EAMMRH LDNAGC RTGHLY RQEAMA SPHRPR KSRPVN GHSGAG SQDEEI LVPPSA 480SSVTAT MAYYYD DGVLSG DETETP RPSTLR REFSDK QWFNTP NKYTKE VIEPAP GHQPVS 540HWVCER EAAVYN AVCNGA LPPPAP FHPLTH GAVPLG STGGPA TGTSSI TSPYNV FAPQGS 600NPKEYR DQQKKV KAKYYH DTNTAG PQSRLL DGLTWD EARRV641<210>26<211>326<212>PRT<213>Schistosoma japonicum<400>26MPIQCR YYEDLF PEVGDV VVVTVK VIQPMG SYVELL EYKNIG GMILHS ELSRRR IRSISK 60LVRIGS NTEVTV VRVDSA KGYIDL SKRRAS AEEIAK CKERFA KAKAVN QILRNV AEKLEY 120ETDEQL EELCRK TAWYFD KKTGRK AGSYDI FKKVVN SPEILD ECDIDQ PTKEML LTDIRH 180RLTPKA VKIRAD FEVSCF TYDGID AVRSAL RSGLKL NSDALP IRINLI APPLYV LTTQTM 240
DRAAGL EQLNEV LNVIQT SIESQG GSFKIR QAPRVV SDTDDA ELQRQM DELEKA NREVSG 300DEDDED DDEDED DDEESN DGDQNE HK 326<210>27<211>103<212>PRT<213>Schistosoma japonicum<400>27MSGRGK GGKGLG KGGAKR HRKVLR DNIQGI TKPAIR RLARRG GVKRIS GLIYEE TRGVLK60VFLENV IRDAVT YTEHAK RKTVTA MDVVYA LKRQGR TLYGFG G 103<210>28<211>417<212>PRT<213>Schistosoma japonicum<400>28MSLTSL YTCRVI VNYHPW SVVYSC SMRCRH YSKGFI QSLIEK VKEESS KDTELK ENIRRF 60REETAK LEQTKE LRSVRE FYKKIE KEMPAD TVDKVR GTFQSV KNKIRN DAQNLA QNETLR 120KGVESL SKAGEH VRDATK SLGETD FVKAAI KSVEVL EKELES DRSKMY QAPSIL KTRSEI 180SGKSED RIMQPD SESSGV VVHKDS KWFTAW QDFKEN NPYVQR FFDLKA NYEESD HLIVRS 240LRFVTD KVSSLF GGFKIN NELQEV LDEVTK MDPSFN LESFIR YCRFVV IPNVLE AIVRVD 300LPILQD WCHEAP FNVLAT PLRQIK ELGYVS ESRILD IHNVDV QMGKIM EQGPVL IITFQA 360QQIHCI RDKNGT VHEGDP NKVLRV THVWAL CRDQSE MHPLAA WRLLDI AMIPSE QWV 417<210>29<211>470<212>PRT<213>Schistosoma japonicum<400>29MAFINL LLRNTG RNNFVR FSPINR SVALSR FEENTL DYDKLE HSLSTV KKRLGH PLTLAE 60KVLYSH LDNPKT ANIERG KSYLML RPDRVA MQDATA QMALLQ FISSGL PRVAVP STVHCD 120HLIEAK DGSETD LNRAND VNKEVY DFLASA SAKYGI GFWKPG SGIIHQ IVLENY AFPGAL 180IIGTDS HTPNGG GLGGLC IGVGGA DAVDVM ANLPWE LKAPLV IGVHLT GKLSGW TSPKDI 240ILKVAE ILTVKG GTGAII EYYGPG VESISC TGMATI CNMGAE IGATTS IFPFND RMLDYL 300RATNRA DIGDLA SKHKAS LLTPDV NCKYDR IIEINL DTLEPH VNGPFT PDLAHP ISKLSS 360HSSKEG WPQYIS AGLIGS CTNSSY EDMSRA ASLARQ ALDKGV KIKSQF FITPGS EQIRAT 420IERDGI TKVFQS VGGIVL ANACGP CIGQWF RKDTKK GDKNTI VSSYNR NF 470
<210>30<211>184<212>PRT<213>Schistosoma japonicum<400>30MSASAL VILSEG AEEMEA VTVADI LARAGV NVTMGG LQGDAI IKGSHG VLIKPN VALSSV 60SSKLFD MVVMPG GMGGSN AMAESK LVGQIL REHEKH GKYIAA ICAAPI ALESHK IAVGKK 120LTSYPG FEDRLR SYTYCE DIVVVD DKLVTS RGPGTA IAFAMK LVELLC SKSKAQ TLAKGM 180LVSL 184<210>31<211>155<212>PRT<213>Schistosoma japonicum<400>31MDVDKA LMEKLE KLKGMS DQVRIG GKGTAR RKKKVV HKNAAA DDKKLQ SSLKKL NLNTIP 60TIEEVN MYKPDG TIIHFK NPKVQA SPQANV FAVSGQ AECKAI NDLLPG VLNQLE SAKLRL 120SKMNEN SKTSDS KIDDGD DDVPDL VEDFDE KSRLE 155<210>32<211>194<212>PRT<213>Schistosoma japonicum<400>32MDFEQE IKLFGK WSVDDL NVNDLS LTDYIN IKRKGN PIVPHT AGRYQV KRFRKA LCPLVE 60RVCCSL MMHGRN NGKKVK AMNILK HTFEII HLLSGE NPIHVL VNAVLN SGARED STRIGR 120GGTVRR QAVDVS PLRRVN QAIYLL CTGARE AAFRNV KTISEC LADELL NAAKGS SNSYAI 180KKKDEL ERVAKS NR 194<210>33<211>223<212>PRT<213>Schistosoma japonicum<400>33MVFRSS MSLVHI LRSSVL RNYGVR FKHTLP PLPYDP SALEPV ISKEIM QLHHSK HHAAYV 60NNLNIA EEQFAD AMSKSD VTKMIS LQPALR FNGGGH INHSIF WHNLSS KGGGVP TGSLAN 120AINDEF GSFDNF KSRLSA TTIAIQ GSGWGW LGYNPN TKRLQI ATCANQ DPLEGT TGLKPL 180LGIDVW EHAYYL QYKNAR PDYVKA IWDIVN WSDVAK RFDLCR N 223
<210>34<211>326<212>PRT<213>Schistosoma japonicum<400>34MAESDK LNIDSL ISRLLE VRGSRP GKNVQL SEGEIR GLALKS REIFLR QPILLE LEAPLK 60ICGDIH GQYYDL LRLFEY GGFPPD ANYLFL GDYVDR GKQSLE TICLLL AYKIKY PENFFL 120LRGNHE CASINR IYGFYD ECKRRY NIKLWK TFTDCF NCLPIV AIVDEK IFCCHG GLSPDL 180HSMEQI RRIMRP TDVPEQ GLLCDL LWSDPD KDITGW GENDRG VSYTFG ADIVTK FLHSHE 240LDLICR AHQVVE DGYEFF AKRQLV TLFSAP NYCGEF DNAGAM MSVDET LTCSFQ ILKPAD 300KKKFNF RSLNSG WPTTPP RNSKLE DK 326<210>35<211>279<212>PRT<213>Schistosoma japonicum<400>35MSGGYP ALELSA EDLRLM LAAHVH VGETNM NFQMST YVHGRT KDNFHI IKLDKT WEKILL 60SARAIA AIDTAS DVFAIG SRPFSQ RAVLKF ANYTGA TAMAGR FTPGAF TNQKQS CFREPR 120LLVVSD PLSDHQ AVMEAS SVNVPL IALCNT DSPLTR VDIVIP CNNKST HSIAVV WWFLAR 180EVRRIR GEDTRL QQWSVL PDLFLY RDPNEE EQNVED PEDARL EPVIPG ADAEAA ETWGVE 240PSMPVG SLGDMG IAAPGY GPVGGT AGGWSN LDADPT ETW 279<210>36<211>302<212>PRT<213>Schistosoma japonicum<400>36MNATIE RMWKEG VSNLSV ISACEL FQRFIT LTTLDT VSFDEC KRYLSE RAQIFI RKVGSC 60RRKIAE NFLNYI PNGSVI FLHSYS RVVLAA LAYAAS AHKRLH CYVTTC SPNGLG AKMTKA 120LAKLKI SCTLVP DNSLAY LMPQVD FVVLGA EAVVES GGILNI LGSSLI AMTASS LGKPVY 180VLAESF KFMRCY PLDQRH IPDEFK WSSDHE YKSLSS PSPLFN LRDKSN CYYFPN DDDDDE 240NKCVFP EMKTVW AAVEAQ RVSVIE QKMPRV DYTSPC YINYLI TDLGVL TPSAVS DELIKL 300YL 302<210>37<211>333
<212>PRT<213>Schistosoma japonicum<400>37MDTVRQ AVTRTA LGLAEY VTPVLK ATKFRE TGVITP EEFVAA GDFLVY HCPTWQ WSIGDK 60PARPYL PKEKQY LVTRSV PCYKRV KQMADH HEEFEK VLEEED GDGGWV DTHHYA TKYGDL 120NPEVEK TNEMSV PSVSHS LKKSNL EETIIS DCEDEE DEDMEA FLQSGM LDECDP AAVKAR 180TKVAND GNTSQA MADFGI DQNIDR ENN6IL QTRTYD LYITYD KYYQTP RLWLFG YDEQHQ 240PLTETQ MYEDFS QDHAKK TVTTEA HPHLSG HMMPSI HPCRQA DVMRKL IEAVAD NGAELA 300VHQYLM VFLKFV QAVIPT IEYDYT RNFNLS SYA 333<210>38<211>281<212>PRT<213>Schistosoma japonicum<400>38MSSLVV DRGPGG FSFDNC YRNKVI EQKGFK VPKAVK TGTTIC GVVFKN GIVLGA DTRATE 60GNIVAE KNCSKI HRIADN IYCCGA GTAADT QMVTLM ISSQVE LHRLNT GRTPRV IAPLRL 120LKRYLY QYQGYV GAALVL GGVDST GPHLFS IAPHGS TDKLPY ITMGSG SLACMS VLESRF 180KFNMEQ DEAVKL VRDGIA AGIFND MGSGSN VDICII TKDGTK YIRSYD EANVKG KRAEKY 240NPPEGT TSVLNK SVHRVE FDVVTT RVVRDM PVHDVE TMDLS281<210>39<211>341<212>PRT<213>Schistosoma japonicum<400>39MFSRGV NVFKFG ITFRSF VTSPKH SLKVAV LGASGG IGQPLS LLLKQS PLIYQL ALYDIA 60HVKGVA ADLSHI ETQAHV TPHLGP GELGEC LSGANL VMIPAG MPRKPG MTRDDL FNTNAS 120IVAELI NACAKN CPKAMI CIITNP VNSTVP IAAEIL KRHNVY DPKRLF GVTTLD VVRSNT 180FIAQAK DLAVRK VSCPVI GGHSGI TILPVI SQCSPH VSFPQD EREKIT KRIQEA GTEVVE 240AKAGAG SATLSM AYAGAR FAISLL EAMNGR AGVVEC AFVQSD VTECEF FSTPLA LGTEGV 300EKNMGI GKLNEY EIELLK KLIPEL QANIKK GKEFAA KFTPK341<210>40<211>216<212>PRT<213>Schistosoma japonicum<400>40
MAGSKW PASVAP TSRVGN TPRAKQ VIFEPT FAKMEK ENYSLT QRLKEA FYKVEK RYPGFS 60KDFMVG LLQRMG VDSEID VTETCL RIAALQ ECDNPR TSRNPR VLELEL TAKTLK TILSSI 120PDEITD RRAFIE VIKGIA DAIKML LAAVGA FYDALP PGTQKK CLEDQK RKFITY CRKFSD 180TLKQYF RNNDQT VYFSSA NLLYNQ TNLILF VVHDVD 216<210>41<211>514<212>PRT<213>Schistosoma japonicum<400>41EVRRLH SIELIG GSSRIP MLKDAV VSIFKQ EGITTL NADEAV ARGCAF QAAICS PAFKVR 60DFNVVE ACLYPI EIKFDR EEGSEQ CMQTEE IGGAIT TQGKAM HIEVFP FLHPIP SSRQIF 120LTRRGA LTLEAR YANTEG LPNGNV VIGTFR IGEASQ MFTEPR KIRLKL RMNTHG IFNISQ 180AQLVEE YEKEVE VSVSED NPVVDG PANSTK EMTTAA NEGNEN PNPSAD NGQVNT QDSTSP 240KKKTVL RKKKFT KYHDLS IEVNNM QFSTKQ LNEFCE ICANLI FKDKLE RERVNA KNAVEE 300YVYEMR SKLQGS LNPFAS PTESES LLQLLD ATEEWL YGDGEC SKRQVY VEKLAE LKNKGD 360PIVHRA NEHLNR PLAVEN FNRSLV RIRKVI DSIAAG EPTYDH LTQEQV NQLENM IVQYET 420WLNQQM MHQNPR PQHADP VVKVTD IISQQQ AMESVC HPIINI PKPTPT SQTNTA DPNSHS 480SEQCNN VNNANT DCNTKS NHSKKC GDNAEN MDLD 514<210>42<211>197<212>PRT<213>Schistosoma japonicum<400>42MAPKAQ PKKQEK STNDPA KKTQDS KKGSKK SQKTSK SLKALK SVKGGK VVKASS KKSAAL 60KALKAQ RAITLG SHTKGK KRVMYT TRFRRP KTYKPA RCPKDR RFLIPK RTKLDA FRIIQH 120PVTTEA AMKKIE DNDTLV FIVDRR ANKPAI KAAVQE LYNIKV AKVNTL NCPNNV KKAYVK 180LPPDFD ALDVAN RIGII 197<210>43<211>355<212>PRT<213>Schistosoma japonicum<400>43YPVQNG ETPKLL YPHVNF LGIPVN KGQPKL GTYQGP DFIRKS NFFQLV AEDGIQ ITDCGD 60VIPVEL SESEDP ERCGMK WSRSFT QTTLKI ADRVEQ LVKGSN KHSIES SNSKPS PLVIVG 120GDHSMA TGTILG HARAKP DVCIIW VDAHGD INTPPN STTGNI HGMPLS FLVKEL QDQIPW 180LDDFHS IKPCLD ASNLVY IGLRDL DVYETR DIRKHG IAYFTM LDVDRM GMEAVI KEALQA 240
VNPRLE KPIHLS FDIDAL DPSIAP STGTAV PGGLTL REGLRI CEEISA TGKLSI VELAEL 300NPLLGS KEDVEK TQSSAV HILRAS LGHCRS GQLPMK VNNSTT NSIVRQ AERIQI K 355<210>44<211>417<212>PRT<213>Schistosoma japonicum<400>44MPTKSP VFTYVI KRLSSH WNHVKL GPPDAI LGITEA YNRDTN PQKINL GAGAYR DDNGKP 60FVLPSV KEAESL LLAKNL NKEYAP ISGIPQ FCDLSI KLALTE QSPRIK DRCNAT VQTISG 120TGALRI GGAFIN EFAEQK HIWMPS PTWGNH IPIFVH SGLNVH QYRYYD SNTCGF DIDGCL 180SDLSKI PKGHFV LLHACA HNPTGV DPSFDQ WRKIGE IMKSRD LIPFFD CAYQGF ASGDIN 240NDAKAI RYFTDE LNFPTL FLTQSF AKNMGL YGERVG AFSLLC SSSDEA ERCLSQ IKILIR 300PMYSNP PIHGAR IAAELM SNVDLR RKWLID LKSMAD RIITMR QSLKNG LIEAGS QHDWSH 360ITNQIG MFCFSG LNPSQV EKLTNE YSIYLT KDGRIS IAGLSS KNVKYL AHAIHQ VTK 417<210>45<211>337<212>PRT<213>Schistosoma japonicum<400>45RLTEEL RSIRDQ TASELE EYKIQM EETFEN KLGQLK SSANFS AEDACR QRSELL MARKRA 60DDLSHD LSKKIA ELELLQ RRVTDL EGQLSD ERRDFE AQLLFQ RQEVER LKEELE ESFREF 120TDLMNT KIALDQ EILMYR KMLEGE ESRLNL TPASRD SPFNIH TVKRRR IDGDYD GDDSTV 180NLTGSS YSSSRS RFAYRV SSTAR6 PVEFFK EQDIHG KWIKIY NSSTEE VNLGSW ELVHEA 240DGHETR FKFSRT FSLKPG TTCTIW SQDAEG NSHNPP SDITMK NKSFHP GTEVTI LLLDAD 300GEEQTK CTVKRE KVRPSV VNFDRK SGVRGH DEKCHL M337<210>46<211>313<212>PRT<213>Schistosoma japonicum<400>46MGRRNS ENNDRR TEQFRK LFIGGL TPLTTE AKLKDF YSQWGE IVDVVV MKDGRT NRSRGF 60GFITYK EPEMVD AAQANR PHEIDG KTVEAK RAMPRE DSNSPE SHMTVT KLFVGG LKKDVT 120HEELRE YFSKYG NITECE VVAWKE SGESRG FGFVTF DDYDPV DKAILY KPHHIG SSRADV 180KKALSK EQINTM KKKQES RIDYNN NYGGGG YGPMGG GYGGYE GGYGGS YGGGPY GGYGSN 240GGGSGG AYGGGN YGGGWG PNHMNG DGPGWG QGGMGD GFGHYG NQQNYA GGPMRG GPPGGY 300QRNAPY GGGYGR R31權利要求
1.一種DNA序列，其編碼表達于日本血吸蟲表皮的蛋白質，其特征在于它具有序列表中SEQ ID NO.1～SEQ ID NO.23所述序列中任一序列的堿基序列；或者它是在中度嚴緊條件下與序列表中SEQ ID NO.1～SEQ ID NO.23所示的序列中任一條序列雜交的序列；或者它是與序列表中SEQ ID NO.1～SEQ ID NO.23所示的序列中任一條序列有至少70％同源性的序列。
2.一種氨基酸序列，其編碼表達于日本血吸蟲表皮的蛋白質，其特征在于它具有序列表中SEQ ID NO.24～SEQ ID NO.46所述序列中任一序列的氨基酸序列；或者它是與序列表中SEQ ID NO.24～SEQ ID NO.46所示的序列中任一條序列有至少70％同源性的序列；或者它是包含有序列表中SEQ IDNO.24～SEQ ID NO.46所示的序列中任一條序列的氨基酸序列。
3.一種從日本血吸蟲中分離獲得日本血吸蟲表皮蛋白質的制備方法。
4.根據權利要求2所述的氨基酸序列在制備血吸蟲免疫蛋白的免疫原方面的應用。
5.根據權利要求2所述的氨基酸序列在篩選化學和其他種類藥物的潛在的藥物的作用靶點方面的應用。
6.根據權利要求2所述的氨基酸序列在制備抗血吸蟲抗體的特異性血吸蟲抗原方面的應用。
7.根據權利要求2所述的氨基酸序列在制備血清學診斷方面需要的蛋白質方面的應用。
8.根據權利要求2所述的核苷酸序列在制備用于基因治療的核苷酸序列方面的應用。
全文摘要
本發明公開了一類在日本血吸蟲表皮上表達的表皮蛋白，尤其公開了所述表皮蛋白的編碼核苷酸和編碼氨基酸。本發明通過大規模的蛋白質組學研究，發現了一類日本血吸蟲表皮蛋白質，這些蛋白質，都是可能的藥物作用靶點和候選疫苗。血吸蟲病的流行給人民身體健康和經濟發展都造成巨大的損失，因此，防治血吸蟲病、開發研制治療血吸蟲病和抗血吸蟲的藥物具有巨大的社會和經濟效益。
文檔編號A61P33/00GK1986810SQ20051011173
公開日2007年6月27日 申請日期2005年12月20日 優先權日2005年12月20日
發明者韓澤廣, 劉鋒, 王志勤, 胡薇, 馮正 申請人:上海人類基因組研究中心, 中國疾病預防控制中心寄生蟲病預防控制所